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KMID : 0545120120220010046
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 1 p.46 ~ p.49
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Identification of the Fur-Binding Site in Regulatory Region of the Vulnibactin-Receptor Gene in Vibrio vulnificus
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Lee Hyun-Jung
Lee Kyu-Ho
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Abstract
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The Vibrio vulnificus vuuA gene, of which expression is repressed by a complex of iron and ferric uptake regulator (Fur), was characterized to localize the Fur-binding site in its upstream regulatory region. In silico analysis suggested the presence of two possible Fur-binding sites; one is a classical Fur-box and the other is a previously reported distinct Fur-binding site. Site-directed mutagenesis and DNase I protection assays revealed the binding site for the iron-Fur complex, which includes an extended inverted repeat containing a homologous sequence to the classical Fur-box.
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KEYWORD
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Fur, vulnibactin-receptor gene, Vibrio vulnificus
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